Escherichia coli (E. coli) is a common bacterium that can cause significant diseases in both humans and animals. The growing threat of antimicrobial resistance (AMR) poses serious risks to public health and food safety, contributing to treatment failures, increased morbidity, and rising healthcare costs. This study, conducted in Chelenko town, Ethiopia, aimed to isolate E. coli and assess its antimicrobial resistance along the beef value chain. The cross-sectional study, carried out from March to September 2022, sought to isolate and identify E. coli in beef samples and evaluate the hygienic practices in abattoirs and butcher shops within Chelenko town, East Hararghe zone, Oromia State, Ethiopia. A total of 384 samples were collected, including 78 beef meat samples, 36 feces samples, 36 water samples, and 234 swab samples from abattoir and butcher staff. Additionally, semi-structured interviews and site observations were used to assess hygienic practices. E. coli was detected in 33 (8.6%) of the total samples, with 16 (7.41%) positive samples originating from abattoirs and 17 (10.12%) from butcher shops. In abattoirs, E. coli was isolated from 2.8% of meat, 2.8% of hand swabs, 2.8% of knife swabs, 19.44% of feces, and 2.8% of water samples. In butcher shops, it was detected in 21.4% of meat, 2.4% of hand swabs, 2.4% of knife swabs, and 11.9% of feces samples. Antimicrobial susceptibility testing revealed high resistance levels, with 97% of isolates resistant to Enrofloxacin, 78.8% to Oxytetracycline and Streptomycin, 72.8% to Tetracycline, and 63.6% to Gentamycin. All E. coli isolates showed complete resistance to Amoxicillin. The study also highlighted poor hygiene practices in both abattoirs and butcher shops, emphasizing the urgent need for improved food safety training and infrastructure to ensure better hygiene in the beef supply chain.
| Published in | European Journal of Clinical and Biomedical Sciences (Volume 10, Issue 4) |
| DOI | 10.11648/j.ejcbs.20241004.12 |
| Page(s) | 57-71 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Abattoir, Beef Meat, Butcheries, Chelenko, Escherichia Coli
| [1] | Mersha, G., Asrat, D., Zewde, B. M., & Kyule, M. (2010). Occurrence of Escherichia coli O157: H7 in faeces, skin and carcasses from sheep and goats in Ethiopia. Letters in applied microbiology, 50(1), 71-76. |
| [2] | Haileselassie, M., Taddele, H., Adhana, K., & Kalayou, S. (2013). Food safety knowledge and practices of abattoir and butchery shops and the microbial profile of meat in Mekelle City, Ethiopia. Asian Pacific journal of tropical biomedicine, 3(5), 407-412. |
| [3] | Carbas, B., Cardoso, L., & Coelho, A. C. (2013). Investigation on the knowledge associated with foodborne diseases in consumers of northeastern Portugal. Food Control, 30(1), 54-57. |
| [4] | Adam, M. and Moss, M. (2008). Food Microbiology 3rd Edition. Royal Society of Chemistry, Cambridge. 216-224 Jores, J., Rumer, L., and Wieler. Impact of the locus of enterocyte effacement pathogenicity island on the evolution of pathogenic Escherichia coli. International Journal of Medical Microbiology. 294, 103-113. |
| [5] | Cobbaut, K., Houf, K., Buvens, G., Habib, I., & De Zutter, L. (2009). Occurrence of non-sorbitol fermenting, verocytotoxin-lacking Escherichia coli O157 on cattle farms. Veterinary microbiology, 138(1-2), 174-178. |
| [6] | Acha, P. N., & Szyfres, B. (2001). Zoonoses and Communicable Diseases Common to Man and Animals: Volume 3: Parasitoses (Vol. 580). Pan American Health Org. |
| [7] | Perelle, S., Dilasser, F., Grout, J., & Fach, P. (2007). Screening food raw materials for the presence of the world's most frequent clinical cases of Shiga toxin-encoding Escherichia coli O26, O103, O111, O145 and O157. International journal of food microbiology, 113(3), 284-288. |
| [8] | Pires, S. M., Majowicz, S., Gill, A., & Devleesschauwer, B. (2019). Global and regional source attribution of Shiga toxin-producing Escherichia coli infections using analysis of outbreak surveillance data. Epidemiology & Infection, 147. |
| [9] | Fratamico, P. M., & Smith, J. L. (2006). Escherichia coli infections. Foodborne infections and intoxications, 205-208. |
| [10] | Pennington, H. (2010). Escherichia coli O157. The Lancet, 376(9750), 1428-1435. |
| [11] | Söderlund, R. (2015). Molecular epidemiology of verotoxigenic Escherichia coli O157: H7 (Vol. 2015, No. 2015: 110). |
| [12] | Pal, M. (2012). Raw meat poses public health risks. The Ethiopian Herald, 68, 2-3. |
| [13] | Humphrey, T., & Jørgensen, F. (2006). Pathogens on meat and infection in animals–Establishing a relationship using Campylobacter and Salmonella as examples. Meat Science, 74(1), 89-97. |
| [14] | Pal, M. 2007. Zoonoses. 2nd Edition. Satyam Publishers, Jaipur, India. 100-134. |
| [15] | Isibor, J. O., Ekundayo, A. O., Ohenhen, R. E., & Orhue, P. O. (2013). Escherichia coli O157: H7-prevalence and risk factors of infection in Edo State, Nigeria. Am J Res Commun, 1(3), 35-49. |
| [16] | Dulo, F., Feleke, A., Szonyi, B., Fries, R., Baumann, M., and Grace, D. 2015. Isolation of multidrug-resistant Escherichia coli O157 from goats in the Somali region of Ethiopia: Across-sectional, abattoir-based study. PloS One, 10, 1–10. |
| [17] | Atnafie, B., Paulos, D., Abera, M., Tefera, G., Hailu, D., Kasaye, S., & Amenu, K. (2017). Occurrence of Escherichia coli O157: H7 in cattle feces and contamination of carcass and various contact surfaces in abattoir and butcher shops of Hawassa, Ethiopia. BMC microbiology, 17, 1-7. |
| [18] | Mengistu, S., Abayneh, E., & Shiferaw, D. (2017). E. coli O157: H7 and Salmonella species: public health importance and microbial safety in beef at selected slaughter houses and retail shops in eastern Ethiopia. J Vet Sci Technol, 8(468), 2. |
| [19] | Taye, M., Berhanu, T., Berhanu, Y., Tamiru, F., & Terefe, D. (2013). Study on carcass contaminating Escherichia coli in apparently healthy slaughtered cattle in Haramaya University slaughter house with special emphasis on Escherichia coli O157: H7, Ethiopia. J Vet Sci Technol, 4(1), 132. |
| [20] | Food and Agricultural Organization. 2019. Technical Guidance Principles of Risk-Based Meat Inspection and their Application; FAO: Rome, Italy. |
| [21] | Ayalew, H., Birhanu, A., & Asrade, B. (2013). Review on food safety system: Ethiopian perspective. Afr J Food Sci, 7(12), 431-40. |
| [22] | Olatoye, I. O., Amosun, E. A., & Ogundipe, G. A. T. (2012). Multidrug-resistant Escherichia coli O157 contamination of beef and chicken in municipal abattoirs of southwest Nigeria. Nature and Science, 10(8), 125-132. |
| [23] | McEvoy, J. M., Doherty, A. M., Sheridan, J. J., Thomson‐Carter, F. M., Garvey, P., McGuire, L.,... & McDowell, D. A. (2003). The prevalence and spread of Escherichia coli O157: H7 at a commercial beef abattoir. Journal of applied microbiology, 95(2), 256-266. |
| [24] | Birke, W., & Zawide, F. (2019). Transforming research results in food safety to community actions: A call for action to advance food safety in Ethiopia. Environ Ecol Res, 7(3), 153-70. |
| [25] | Ahmad, M. U. D., Sarwar, A., Najeeb, M. I., Nawaz, M., Anjum, A. A., Ali, M. A., & Mansur, N. (2013). Assessment of microbial load of raw meat at abattoirs and retail outlets. J. Anim. plant sci, 23(3), 745-748. |
| [26] | Eliyas, A. (2023). Knowledge, Attitude, Hygiene Practices, and Enumeration of Salmonela from Raw Meat at Retailer Shops in Chelenko Town, Eastern Ethiopia. |
| [27] | Thrusfield, M. (2007). Sample size determination. Veterinary epidemiology, 3, 185-189. |
| [28] | Gallina, S., Bianchi, D. M., Ru, G., Maurella, C., Barzanti, P., Baioni, E.,... & Decastelli, L. (2015). Microbiological recovery from bovine, swine, equine, and ovine carcasses: Comparison of excision, sponge and swab sampling methods. Food control, 50, 919-924. |
| [29] | Timothy M, and Smith JR. (2012). Isolation, Identification, and Enumeration of Pathogenic Salmonella Serovars from Environmental Waters. J. Food Prot, 2-251. |
| [30] | Megersa, R., Mathewos, M., & Fesseha, H. (2019). Isolation and Identification of Escherichia coli from dairy cow raw milk in Bishoftu Town, Central Ethiopia. Arch Vet Anim Sci, 1(1). |
| [31] | Wayne, P. A. (2010). Clinical and Laboratory Standards Institute: Performance standards for antimicrobial susceptibility testing: 20th informational supplement. CLSI document M100-S20. |
| [32] | Chitra, S. R. (2017). Theoretical Investigation on Antimicrobial Susceptibility Testing Methods. Bioinformatics, 5(2), 12-26. |
| [33] | Hall, D. A., Zaragoza Domingo, S., Hamdache, L. Z., Manchaiah, V., Thammaiah, S., Evans, C.,... & International Collegium of Rehabilitative Audiology and TINnitus Research NETwork. (2018). A good practice guide for translating and adapting hearing-related questionnaires for different languages and cultures. International Journal of Audiology, 57(3), 161-175. |
| [34] | Mesfine, S., Feyera, T., & Mohammed, O. (2015). Microbiological quality of raw cow’s milk from four dairy farms in Dire Dawa City, Eastern Ethiopia. World Journal of Dairy & Food Sciences, 10(1), 09-14. |
| [35] | Guzewich, J., & Ross, M. P. (1999). Evaluation of risks related to microbiological contamination of ready-to-eat food by food preparation workers and the effectiveness of interventions to minimize those risks. Food and Drug Administration: Centre for Food safety and Applied Nutrition. Retrieved September, 20, 2007. |
| [36] | Abdi Hassen, K., Girma, D. S., Gumi, D. B., & Zerihun, D. T. (2019). Assessment On Hygienic Practice of Camel Meat Han-Dling, And Identifying the Main Source of Bacterial Contamination in Abattoir and Butcheries of Nagelle Town, Southern Oromia, Ethiopia (Doctoral dissertation, Haramaya university). |
| [37] | Koffi-Nevry, R., Koussemon, M., & Coulibaly, S. O. (2011). Bacteriological quality of beef offered for retail sale in Cote d’ivoire. American Journal of Food Technology, 6(9), 835-842. |
| [38] | Egan, M. B., Raats, M. M., Grubb, S. M., Eves, A., Lumbers, M. L., Dean, M. S., & Adams, M. R. (2007). A review of food safety and food hygiene training studies in the commercial sector. Food control, 18(10), 1180-1190. |
| [39] | Adetunde, L. A., Glover, R. L. K., Oliver, A. W. O., & Samuel, T. (2011). Source and distribution of microbial contamination on beef and Chevron in Navrongo, Kassena Nankana district of Upper East region in Ghana. Journal of Animal Production Advances, 1(1), 21-28. |
| [40] | Bersisa, A., Tulu, D., & Negera, C. (2019). Investigation of bacteriological quality of meat from abattoir and butcher shops in Bishoftu, Central Ethiopia. International journal of microbiology, 2019. |
| [41] | Bhattarai, J., Badhu, A., Shah, T., & Niraula, S. R. (2017). Meat hygiene practices among meat sellers in dharan municipality of eastern Nepal. Birat Journal of Health Sciences, 2(2), 184-190. |
| [42] | Sallami, Z. A. (2016). Assessment of hand hygiene attitude, knowledge and practice among health science students in aden university. Journal of Biosciences and Medicines, 4(9), 25-32. |
| [43] | Hogan Eamonn, Alan L. Kelly, Da Wen Sun. (2005). “High Pressure Processing of Foods. An Overview.” Emerging Technologies for Food Processing, 3–32. |
| [44] | Zelalem, A., Abegaz, K., Kebede, A., Terefe, Y., Schwan, C. L., & Vipham, J. L. (2021). Food Safety Knowledge, Attitudes, and Hygienic Practices of Abattoir Workers in Ethiopia: A Cross-Sectional Study. Food Protection Trends, 41(5). |
| [45] | Seifu, B., & Sentayhu, M. (2017). Microbial quality of retail raw meat in administrative towns of Gojjam area North-West Ethiopia with special reference of Gram positive cocci species. African Journal of Microbiology Research, 11(42), 1538-1543. |
| [46] | Reid, C. A., Small, A., Avery, S. M., & Buncic, S. (2002). Presence of food-borne pathogens on cattle hides. Food control, 13(6-7), 411-415. |
| [47] | Mohammed O., D. Shimelis, P. Admasu, and T. Feyera, 2014. “Prevalence and antimicrobial susceptibility pattern of E. coli isolates from raw meat samples obtained from abattoirs in Dire Dawa city, eastern Ethiopia,” International Journal of Microbiological Research, 5, 35–39. |
| [48] | Debebe, G., Girima, S., & Take, W. (2022). Assessment of Hygienic Practice, Isolation and Identification of Escherichia Coli from Beef Meat and Environmental Sample at Wolaita Sodo Municipal Abattoir and Butchery Shop, Southern, Ethiopia (Doctoral dissertation, Haramaya University). |
| [49] | Hiko, A., Asrat, D., & Zewde, G. (2008). Occurrence of Escherichia coli O157in retail raw meat products in Addis Ababa, Ethiopia. Journal of Infection in Developing Countries, 2(5), 389-393. |
| [50] | Sebsibe, M. A., & Asfaw, E. T. (2020). Occurrence of multi-drug resistant Escherichia coli and Escherichia coli O157: H7 in meat and swab samples of various contact surfaces at abattoir and butcher shops in Jimma town, Southwest district of Ethiopia. Infection and Drug Resistance, 3853-3862. |
| [51] | Akanbi BO, Mbah IP, Kerry PC. 2011. Prevalence of Escherichia coli O157:H7 on hides and faeces of ruminants at slaughter in two major abattoirs in Nigeria. Lett Appl Microbiol. 53, 336-40. |
| [52] | Rahman M, Rahman A, Islam M, Alam M. 2017. Antimicrobial Resistance of Escherichia Coli Isolated from Milk, Beef and Chicken Meat in Bangladesh. Bangl. J. Vet. Med. 15(2), 141-146. |
| [53] | Polpakdee, A., & Angkititrakul, S. (2015). Prevalence of Staphylococcus aureus, Escherichia coli and Salmonella spp. isolated from meat and cooked meat at Khon Kaen Municipality Schools. Antimicrobial Resistance and Infection Control, 4(Suppl 1), P114. |
| [54] | Soepranianondo, K., Wardhana, DK., Budiarto and Diyantoro. 2019. Analysis of bacterial contamination and antibiotic residue of beef meat from city slaughterhouses in East Java Province, Indonesia. Veterinary World, 12, 243-248. |
| [55] | Chaudhary, S., Khurana, S. K., & Mane, B. G. (2014). Escherichia coli: animal foods and public health-review. Journal of Microbiology, Immunology and Biotechnology, 1, 31-46. |
| [56] | Jaja, I. F., Bhembe, N. L., Green, E., Oguttu, J., & Muchenje, V. (2019). Molecular characterisation of antibiotic-resistant Salmonella enterica isolates recovered from meat in South Africa. Acta Tropica, 190, 129-136. |
| [57] | Barka, M. S., & Kihal, M. (2010). Prevalence of Escherichia coli enterohemorragic O157: h7 in frozen bovine meat in Algeria. Journal of Applied Sciences Research, (November), 1576-1580. |
| [58] | Guyon, R., Dorey, F., Malas, J. P., & Leclercq, A. (2001). Hazard analysis of Escherichia coli O157: H7 contamination during beef slaughtering in Calvados, France. Journal of food protection, 64(9), 1341-1345. |
| [59] | Hajian S., E. Rahimi, and H. Mumtaz, 2011. “A 3-year study of Escherichia coli O157:H7 in cattle, camel, sheep, goat, chicken and beef minced meat,” Food Engineering and Biotechnology, vol. 9: 162–166. |
| [60] | Itelima J. U. and S. E. Agina. 2011. The occurrence of Escherichia coli O157:H7 in market and abattoir meat in plateau state, Nigeria. Global Journal of Environmental Sciences 10: 47-55. |
| [61] | Kago, J. M. (2015). Assessment of beef carcass contamination with escherichia coli 0157: h7 post slaughter in Kenya (Doctoral dissertation, University of Nairobi). |
| [62] | Hassan AN, Farooqui A, Khan A, Khan Y, Kazmi SU. 2010. Microbial contamination of raw meat and its environment in retail shops in Karachi, Pakistan. J. Infect. Dev. Ctries. 4(6): 382-388. |
| [63] | Sharafat, S., Kalhoro, D. H., Kalhoro, M. S., Abro, S. H., Mangi, M. H., Laghari, A. A.,... & Hussain, M. (2023). Prevalence and Antimicrobial Resistance of Staphylococcus aureus, Salmonella and Escherichia coli Isolated from Poultry Meat in Tandojam, Hyderabad, Pakistan. |
| [64] | Siriken, B., & Pamuk, S. (2004). Investigation of the incidence of E. coli O157: H7 and L. monocytogenes from ground beef sold in Afyon district. In I. National Veterinary Food Hygiene Congress (pp. 101-109). |
| [65] | Bonomo, M. G., Ricciardi, A., & Zotta, T. (2007). Prevalence of Escherichia coli O157 in ground beef in Italy. Italian Journal of Food Science, 19(2), 209-214. |
| [66] | Burush, I., Bayu, Y., & Wondmu, A. (2018). Study on Isolation and Identification of E. coli from Carcass of Slaughtered Goat and Environmental Samples at Selected Abattoirs, Ethiopia. International Journal of Microbiological Research, 9, 01-06. |
| [67] | Scott, K. P. (2002). The role of conjugative transposons in spreading antibiotic resistance between bacteria that inhabit the gastrointestinal tract. Cellular and Molecular Life Sciences CMLS, 59, 2071-2082. |
| [68] | Callaway, T. R., Anderson, R. C., Edrington, T. S., Elder, R. O., Genovese, K. J., Bischoff, K. M.,... & Nisbet, D. J. (2003). Preslaughter intervention strategies to reduce food-borne pathogens in food animals. Journal of Animal Science, 81(14_suppl_2), E17-E23. |
| [69] | Mashood, A. R., Uswege, M., & Robert, M. (2006). Current epidemiological status of enterohaemorrhagic Escherichia coli O157:H7 in Africa. Chinese Medical Journal, 119(03), 217-222. |
| [70] | Khachatourians, G. G. (1998). Agricultural use of antibiotics and the evolution and transfer of antibiotic-resistant bacteria. Cmaj, 159(9), 1129-1136. |
| [71] | Institute of Food Technologists. (2002). IFT expert report on emerging microbiological food safety issues: implications for control in the 21st century. |
| [72] | Davies, J., & Davies, D. (2010). Origins and evolution of Antibiotic Resistance. Microbiology and Molecular Biology Reviews, 74(3), 417–433. |
| [73] | Shecho, M., Thomas, N., Kemal, J., & Muktar, Y. (2017). Cloacael carriage and multidrug resistance Escherichia coli O157: H7 from poultry farms, eastern Ethiopia. Journal of veterinary medicine, 2017. |
| [74] | Welde, N., Abunna, F., & Wodajnew, B. (2020). Isolation, identification and antimicrobial susceptibility profiles of E. coli O157: H7 from raw cow milk in and around Modjo Town, Ethiopia. J Am Sci, 16(6), 62-79. |
| [75] | Mora, A., Blanco, J. E., Blanco, M., Alonso, M. P., Dhabi, G., Echeita, A.,... & Blanco, J. (2005). Antimicrobial resistance of Shiga toxin (verotoxin)-producing Escherichia coli O157: H7 and non-O157 strains isolated from humans, cattle, sheep and food in Spain. Research in microbiology, 156(7), 793-806. |
| [76] | Srinivasan, V., Nguyen, L. T., Headrick, S. I., Murinda, S. E., & Oliver, S. P. (2007). Antimicrobial resistance patterns of Shiga toxin-producing Escherichia coli O157: H7 and O157: H7− from different origins. Microbial Drug Resistance, 13(1), 44-51. |
| [77] | Tassew, A. (2015). Isolation, Identification, Antimicrobial Profile and Molecular Characterization of Enterohaemorrhagic E. coli O157: H7 Isolated from Ruminants Slaughtered at Debre Zeit ELFORA Export Abattoir and Addis Ababa Abattoirs Enterprise. Journal of Veterinary Sci. Techno, 6, 2-13. |
| [78] | Guerra, B., Junker, E., Schroeter, A., Malorny, B., Lehmann, S., & Helmuth, R. (2003). Phenotypic and genotypic characterization of antimicrobial resistance in German Escherichia coli isolates from cattle, swine and poultry. Journal of Antimicrobial Chemotherapy, 52(3), 489-492. |
| [79] | Zhao, S., Maurer, J. J., Hubert, S., De Villena, J. F., McDermott, P. F., Meng, J.,... & White, D. G. (2005). Antimicrobial susceptibility and molecular characterization of avian pathogenic Escherichia coli isolates. Veterinary microbiology, 107(3-4), 215-224. |
| [80] | Akond, M. A., Alam, S., Hassan, S. M. R., & Shirin, M. (2009). Antibiotic resistance of Escherichia coli isolated from poultry and poultry environment of Bangladesh. Internet Journal of food safety, 11, 19-23. |
| [81] | Sharada, R., & Ruban, S. W. (2010). Isolation, characterization and antibiotic resistance pattern of Escherichia coli isolated from poultry. American-Eurasian Journal of Scientific Research, 5(1), 18-22. |
| [82] | Majalija, S., Francis, O., Sarah, W. G., Vudriko, P., & Nakamya, F. M. (2010). Antibiotic susceptibility profiles of fecal Escherichia coli isolates from dip-litter broiler chickens in Northern and Central Uganda. Veterinary Research (Pakistan), 3(4), 75-80. |
| [83] | Reuben, R. C., & Owuna, G. (2013). Antimicrobial resistance patterns of Escherichia coli O157: H7 from Nigerian fermented milk samples in Nasarawa State, Nigeria. International Journal of Pharmaceutical Science Invention, 2(3), 38-44. |
| [84] | Marshall, B. M., & Levy, S. B. (2011). Food animals and antimicrobials: Impacts on human health. Clinical Microbioogy Reviews, 24(4), 718–733. |
APA Style
Mekonnen, A. S., Mumed, B. A., Dawed, A. (2024). Food Safety, Isolation and Antibiogram Escherichia Coli Along Beef Value Chain in Chelenko Town, Eastern Ethiopia. European Journal of Clinical and Biomedical Sciences, 10(4), 57-71. https://doi.org/10.11648/j.ejcbs.20241004.12
ACS Style
Mekonnen, A. S.; Mumed, B. A.; Dawed, A. Food Safety, Isolation and Antibiogram Escherichia Coli Along Beef Value Chain in Chelenko Town, Eastern Ethiopia. Eur. J. Clin. Biomed. Sci. 2024, 10(4), 57-71. doi: 10.11648/j.ejcbs.20241004.12
AMA Style
Mekonnen AS, Mumed BA, Dawed A. Food Safety, Isolation and Antibiogram Escherichia Coli Along Beef Value Chain in Chelenko Town, Eastern Ethiopia. Eur J Clin Biomed Sci. 2024;10(4):57-71. doi: 10.11648/j.ejcbs.20241004.12
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Download@article{10.11648/j.ejcbs.20241004.12,
author = {Abnet Shewafera Mekonnen and Bayan Ahmed Mumed and Abrahim Dawed},
title = {Food Safety, Isolation and Antibiogram Escherichia Coli Along Beef Value Chain in Chelenko Town, Eastern Ethiopia
},
journal = {European Journal of Clinical and Biomedical Sciences},
volume = {10},
number = {4},
pages = {57-71},
doi = {10.11648/j.ejcbs.20241004.12},
url = {https://doi.org/10.11648/j.ejcbs.20241004.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejcbs.20241004.12},
abstract = {Escherichia coli (E. coli) is a common bacterium that can cause significant diseases in both humans and animals. The growing threat of antimicrobial resistance (AMR) poses serious risks to public health and food safety, contributing to treatment failures, increased morbidity, and rising healthcare costs. This study, conducted in Chelenko town, Ethiopia, aimed to isolate E. coli and assess its antimicrobial resistance along the beef value chain. The cross-sectional study, carried out from March to September 2022, sought to isolate and identify E. coli in beef samples and evaluate the hygienic practices in abattoirs and butcher shops within Chelenko town, East Hararghe zone, Oromia State, Ethiopia. A total of 384 samples were collected, including 78 beef meat samples, 36 feces samples, 36 water samples, and 234 swab samples from abattoir and butcher staff. Additionally, semi-structured interviews and site observations were used to assess hygienic practices. E. coli was detected in 33 (8.6%) of the total samples, with 16 (7.41%) positive samples originating from abattoirs and 17 (10.12%) from butcher shops. In abattoirs, E. coli was isolated from 2.8% of meat, 2.8% of hand swabs, 2.8% of knife swabs, 19.44% of feces, and 2.8% of water samples. In butcher shops, it was detected in 21.4% of meat, 2.4% of hand swabs, 2.4% of knife swabs, and 11.9% of feces samples. Antimicrobial susceptibility testing revealed high resistance levels, with 97% of isolates resistant to Enrofloxacin, 78.8% to Oxytetracycline and Streptomycin, 72.8% to Tetracycline, and 63.6% to Gentamycin. All E. coli isolates showed complete resistance to Amoxicillin. The study also highlighted poor hygiene practices in both abattoirs and butcher shops, emphasizing the urgent need for improved food safety training and infrastructure to ensure better hygiene in the beef supply chain.
},
year = {2024}
}
TY - JOUR T1 - Food Safety, Isolation and Antibiogram Escherichia Coli Along Beef Value Chain in Chelenko Town, Eastern Ethiopia AU - Abnet Shewafera Mekonnen AU - Bayan Ahmed Mumed AU - Abrahim Dawed Y1 - 2024/11/29 PY - 2024 N1 - https://doi.org/10.11648/j.ejcbs.20241004.12 DO - 10.11648/j.ejcbs.20241004.12 T2 - European Journal of Clinical and Biomedical Sciences JF - European Journal of Clinical and Biomedical Sciences JO - European Journal of Clinical and Biomedical Sciences SP - 57 EP - 71 PB - Science Publishing Group SN - 2575-5005 UR - https://doi.org/10.11648/j.ejcbs.20241004.12 AB - Escherichia coli (E. coli) is a common bacterium that can cause significant diseases in both humans and animals. The growing threat of antimicrobial resistance (AMR) poses serious risks to public health and food safety, contributing to treatment failures, increased morbidity, and rising healthcare costs. This study, conducted in Chelenko town, Ethiopia, aimed to isolate E. coli and assess its antimicrobial resistance along the beef value chain. The cross-sectional study, carried out from March to September 2022, sought to isolate and identify E. coli in beef samples and evaluate the hygienic practices in abattoirs and butcher shops within Chelenko town, East Hararghe zone, Oromia State, Ethiopia. A total of 384 samples were collected, including 78 beef meat samples, 36 feces samples, 36 water samples, and 234 swab samples from abattoir and butcher staff. Additionally, semi-structured interviews and site observations were used to assess hygienic practices. E. coli was detected in 33 (8.6%) of the total samples, with 16 (7.41%) positive samples originating from abattoirs and 17 (10.12%) from butcher shops. In abattoirs, E. coli was isolated from 2.8% of meat, 2.8% of hand swabs, 2.8% of knife swabs, 19.44% of feces, and 2.8% of water samples. In butcher shops, it was detected in 21.4% of meat, 2.4% of hand swabs, 2.4% of knife swabs, and 11.9% of feces samples. Antimicrobial susceptibility testing revealed high resistance levels, with 97% of isolates resistant to Enrofloxacin, 78.8% to Oxytetracycline and Streptomycin, 72.8% to Tetracycline, and 63.6% to Gentamycin. All E. coli isolates showed complete resistance to Amoxicillin. The study also highlighted poor hygiene practices in both abattoirs and butcher shops, emphasizing the urgent need for improved food safety training and infrastructure to ensure better hygiene in the beef supply chain. VL - 10 IS - 4 ER -
College of Veterinary Medicine, Haramaya University, Dire Dawa, Ethiopia;Veterinary Drug and Feed Administration and Control Authority (VDFACA), Ethiopian Minster of Agriculture, Addis Ababa, Ethiopia
College of Veterinary Medicine, Haramaya University, Dire Dawa, Ethiopia
Veterinary Drug and Feed Administration and Control Authority (VDFACA), Ethiopian Minster of Agriculture, Addis Ababa, Ethiopia
