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Risk Analysis and Antibiogram Spectrum of Escherichia coli O157: H7 Serotype from Children Stool and Raw Bovine Meat in Households Across Cross River State, Nigeria

Received: 9 June 2018     Accepted: 24 July 2018     Published: 24 August 2018
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Abstract

This cross sectional study is aimed at evaluating the risk factors and antibiogram profiles of Escherichia coli O157:H7 in children stool and bovine meat obtained from selected households across Cross River State, Southern Nigeria. A total of 360 samples of fresh household bovine meat and 366 children diarrheal and nondiarrheal stool samples each were collected and examined for E. coli O157: H7 using standard culture and serological methods. Confirmed E. coli O157: H7 isolates were evaluated for antimicrobial susceptibility using the Agar disc diffusion method. The total positive samples for E. coli O157: H7 in household meat was 76/360 (21.11%,) while the diarrheaic and nondiarrheaic stool samples had 70/366 (19.13%) and 5/366 (1.37%) positive samples respectively. A significant difference was observed in the prevalence values among the bovine meat samples from various households and between the diarrheaic and nondiarrheaic samples at p<0.05. Risk factors such as Age range with highest prevalence value at 1-2yrs (26.83%); Occupation of parent/guardian with highest value from farming (25.67%) and Main domestic water source with highest value from surface water (28.21%) were observed to significantly affect the prevalence of the pathogen in children diarrheaic stool (p<0.05). All 70 diarrheaic isolates were resistant to one or multiple antibiotics with highest values obtained from tetracycline (88.6%) and cotrimoxazole (77.1%). This study revealed that bovine meat and some human and environmental factors play a vital role in the establishment of E. coli O157:H7 infection in children in the study communities with diarrheal stool being the main vehicle for secondary infections in humans. Cattle therefore serve as a major source of transmission of multi drug resistant E. coli O157:H7 to humans hence the need for continuous surveillance of this pathogen and implementation of legislation against indiscriminate use of antibiotics in diary farms.

Published in European Journal of Clinical and Biomedical Sciences (Volume 4, Issue 3)
DOI 10.11648/j.ejcbs.20180403.11
Page(s) 39-45
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Escherichia coli O157:H7, Children Stool, Bovine Meat, Risk Factors, Antibiotics, Nigeria

References
[1] Griffin, P. M. (1995). Escherichia coli O157:H7 and other Enterohemorrhagic Escherichia coli, P. 739-761. In M. J. Blaser, P. D. Smith, J. I. Rovin, H. B. Greenberg, And R. L. Guerrant (Ed.), Infections of the Gastrointestinal Tract. Raven Press, New York, NY
[2] Seibt, F., Filer, G., Gellermann, J., Beutin, L., Ehrih, J. (1995). The Heterogeneity of Haemolytic Uraemic Syndromes in Children and Adults. Nephrologisches Jahresgespräch. Editor: Deutsche Dialysegesellschaft.
[3] Mora, A., Blanco, J. E. and Blanco, M. (2005). Antimicrobial resistance of Shiga toxin (verotoxin)- producing Escherichia coli O157:H7 and non-O157 strains isolated from humans, cattle, sheep and food in Spain. Research in Microbiology, 156(7): 793–806.
[4] Mead, P. S. and Griffin, P. M. (1998). Escherichia coli O157:H7. Lancet, (35): 1207-1212.
[5] Cooley, M. (2007). Incidence and Tracking of Esherichia coli O157:H7 in a major produce production region in California. PLos ONE, 2(11): e1159.
[6] Enem, S. I. and Oboegbulem, S. I. (2015). Epidemiology of Verocytotoxigenic Escherichia coli (VTEC) O157 Serotype in Cattle in Federal Capital Territory, Abuja, Nigeria. Journal of Veterinary Medicine and Research, 2(3): 10-26.
[7] Duffy, G. (2006). Emerging pathogenic E. coli. In: Emerging Foodborne Pathogens, M. Yasmine and A. Martin, Eds. Pp. 253–272, CRC.
[8] Presterl, E., Zuick, R. H., Reichmann, S., Aichelburg, A., Winkler, S., Kremsner, P. G and Graninger, A(2003). Frequency and Virulence Properties of Diarrhoeagenic Escherichia coli in Children with Diarrhoea in Gabon. American Journal of Tropical Medicine and Hygiene. 69(4):406-410.
[9] Nigatu, D., Berhanu, S., Shimelis, M., Yimer, M., and Dinaol, B. (2017). Prevalence and Antimicrobial Susceptibility Pattern of E. coli O157:H7 Isolated from Traditionally Marketed Raw Cow Milk in and around Asosa Town, Western Ethiopia. Veterinary Medicine International, (7): 1-7.
[10] Galland J C. (2001). Prevalence, antibiotic susceptibility, and diversity of Escherichia coli O157:H7 isolates from a longitudinal study of beef cattle feedlots. Applied and Environmental Microbiology, 67(4):1619-1627.
[11] Raji, M. A., Minga, U. and Machangu, R. (2006). Current epidemiological status of enterohaemorrhagic Escherichia coli O157:H7 in Africa,” Chinese Medical Journal, 119(3): 217–222
[12] Walsh, C., Duffy, G., O’Mahony, R., Fanning, S., Blair, I. S. and McDowell, D. A. (2006). Antimicrobial resistance in Irish isolates of verocytotoxigenic Escherichia coli (E. coli)-VTEC. International Journal of Food Microbiology, 109(3): 173–178.
[13] Reuben, C. R. and Gyar, S. D. (2015). Isolation and Antibiogram of Shiga Toxin-Producing Escherichia coli O157:H7 from Diarrhoeic HIV/AIDS Patients in Lafia, Central Nigeria. International Research Journal of Microbiology, 6(2): 020-026,
[14] Meng, J. and Doyle, M. P. (1998). Emerging and evolving microbial foodborne pathogens. Bulletin de L’Institut Pasteur, 96: 151-164.
[15] Molbak, K., Mead, P. S. and Griffin, P. M. (2002). Antimicrobial Therapy in Patients with Escherichia coli O157:H7 Infection. Journal of American Medical Association, 288:1014-6.
[16] Nfongeh J. F; Epoke, J; Antai, E. E; Ikpeme, E. M; Etim, L. B; Akeh, M. and Ekpiken, S. E. (2014). The Effects of Escherichia coli 0157:H7 lipopolysaccharide (LPS) from human, cattle and poultry isolates on haematological parameters of neonatal albino rats. European Journal of Experimental Biology 4(1):538-542
[17] Clinical and Laboratory Standard Institute (CLSI) (2007). Performance Standards for Antimicrobial Susceptibility Testing. Seventeenth Informational Supplement. Approved Standard M100-S17: Wayne, PA.
[18] Hiko, A., Asrat, D. and Zewde, G. (2008). Occurrence of Escherichia coli O157:H7 in retail raw meat products in Ethiopia. J Infect Dev Ctries., 2(5):389–393
[19] Tizeta, B., Girma, Z., Genene, T., Aklilu, F. and Kaleab, Z. (2014). Escherichia coli O157:H7 in Raw Meat in Addis Ababa, Ethiopia: Prevalence at an Abattoir and Retailers and Antimicrobial Susceptibility. International Journal of Food Contamination, 1(4): 3-8
[20] Abong’O, B. O. (2008). Prevalence of Escherichia coli 0157:H7 in water, meat, meat products and vegetables sold in the Eastern Cape Province of South Africa and its impact on the diarrhoeic conditions of HIV/AIDS patients. PhD Thesis University of Port Hare, South Africa.
[21] Hajian, S., Rahim, E. & Mommtaz, H., (2011). A 3-year study of E. coli 0157:H7 in cattle, camel, sheep, goat, chicken and beef minced meat. International Conference on Food Engineering and Biotechmology. 9: 162-166
[22] Dahiru, M., Uraih, N., Enabulele, S. A. and Shamsudeen, U. (2008). Prevalence of Escherichia coli 0157:H7 in fresh and roasted beef in Kano City, Nigeria. Bayero J Pure Appl Sci., 1:39–42
[23] McEvoy, J. M., Sheridan, J. J. and McDowell, D. A. (2004). Major pathogens associated with the processing of beef. In: Collins JD (ed) Smulders FJM. Wageningen Academic Publishers, Safety Assurance during Food Processing, pp 57–80.
[24] Fard, A. H., Bokaeian, M. and Qureishi, M. E. (2008). Frequency of Escherichia coli O157: H7 in children with diarrhoea in Zahedan, Islamic Republic of Iran. East Mediterr Health J., 14:1022–27
[25] . Abdulaziz, H. O., Aminu, M. and Machido, D. A. (2016). Isolation and Characterisation of Esherichia coli O157 in Human Stool Samples from Parts of Kaduna Metropolis Nigeria. American Journal of Food Science and Technology, 4(5): 125-128.
[26] Olorunshola, I. D., Smith, S. I. and Coker, A. O. (2000). Prevalence of Enterohaemorrhagic Escherichian coli in Patients with Diarrhoea in Lagos, Nigeria. Actapathologica, microbiologica, etimmunologica Scandinavica, 108(11):761-763.
[27] Esumeh, F. I, Isibor, J. O., Egbagbe, I. D. S. (2011). Screening For Escherichia Coli O157:H7 In Diarrheic Patients In Benin City, Nigeria. Journal of Microbiology and Biotechnology Research, 1(4): 1-4.
[28] Opintan, J. A., Bishar, R. A., Newman, M. J and Okeke, I. N. (2010). Carriage of diarrheagenic Escherichia coli by older children and adults in Accra Ghana. Trans R Soc Trop Med Hyg., 104(7): 504-506.
[29] Umolu, P. I., Omigie, O., Tatfeng, Y., Omorogbe, F. I., Aisabokhale, F. and Ugbodagah, O. P. (2006). Antimicrobial susceptibility and plasmid profiles of Escherichia coli isolates obtained from different human clinical specimens in Lagos – Nigeria. American Journal of Science, 2(4): 70-76.
[30] Lim, J. Y., Yoon, J and Hovde, C. J. (2010). A brief overview of Escherichia coli O157:H7 and its plasmid O157. J. Microbiol. Biotechnol., 20 (1):5-14.
[31] Marsh, J., MacLeod, A. F., Hanson, M. F., Emmanuel, F. X. S, Frost, J. A and Thomas, A. A. (1992). A restaurant-associated outbreak of E coli O157infection. J. Publ. Health Med., 14: 78-83.
[32] WHO. (2000). Global Principles for the Containment of Antimicrobial Resistance in Animals Intended For Food; Report of WHO Consultation With The Participation Of Food and Agriculture Organization of The United Nation and the Office International Des Epizooties, Geneva Switzerland 5- 9 June 2000. Department of Communicable Disease Surveillance and Response.
[33] Galland, C. J., Hyatt, R. D., Crupper, S. S. and Acheson, W. D. (2001). Prevalence, Antibiotic Susceptibility, and Diversity of E. coli O157:H7 Isolates from a Longitudinal Study of Beef Cattle Feedlots. Journal of Applied and Environmental Microbiology, 67(4):1619-27.
[34] Olatoye, I. O. (2010). The Incidence and Antibiotics Susceptibility of Escherichia coli O157:H7 from Beef in Ibadan Municipal, Nigeria. African Journal of Biotechnology, 9(8): 1196-1199.
[35] AlHaj, N., Mariana, N. S., Raha, A. R. and Ishak, Z. (2007). Prevalence of Antibiotic Resistance Among Escherichia coli from Different Sources. Malaysia Research Journal of Pharmacology, 1(2): 44-49.
[36] Helali, A. (2002). Pharmacologie Fondamentale Et Clinique A L’usage Des Etudiants En Médecine, Santé Collection, ENAG/ Editions. Pp 183.
[37] Naik, J. and Desai, P. (2012). Detection of Enterohaemorrhagic Escherichia coli (E. coli O157:H7) and its Drug Resistance Pattern. Journal of Environmental Research and Development, 7(1): 51-55
[38] Daini, O. A., Ogbolu, O. D. and Ogunledun, A. (2005). Quinolones resistance and R- Plasmids of some Gram-negative enteric bacilli. African Journal of Clinical and Experimental Microbiology, 6(1): 14-20.
[39] Oteo, J., Lazaro, E., de Abjo, F. J., Baquero, F. and Campos, J. (2005). Spanish members of EARSS. Antimicrobial-resistant invasive Escherichia coli, Spain. Emerging Infectious Diseases, 11(4): 546- 553.
[40] Isibor, J. O., Erhunmwuns, P. I. and Nwobu, G. O. (2003). Antimicrobial susceptibility of clinical isolates of E. coli to six antimicrobial agents. Journal of Applied Basic Science, 1(2):37-40.
[41] Schroeder, C. M., Zhao, C., DebRoy, C., Torcolini, J., Zhao, S., White, D. G., Wagner, D. D., McDermott, P. F., Walker, R. D., and Meng, J. (2002). Antimicrobial Resistance of Escherichia coli O157 Isolated from Humans, Cattle, Swine, and Food. Appl. Environ. Microbiol. 68 (2): 576–581
[42] Reuben, C. R. and Owuna, G. (2013). Antimicrobial Resistance Patterns of Escherichia coli O157:H7 from Nigerian Fermented Milk Samples in Nasarawa State, Nigeria. Int. J. Pharmaceutical Sci. Invent., 2(3): 38-44.
[43] Mincey, B. A. and Parkulo, M. A. (2001). Antibiotic prescribing practices in a teaching clinic: comparison of resident and staff physicians. Southern Med. J., 94(4): 365 - 369.
[44] Granizo, J. J., Aguilar, L., Casal, J., Dal-Re, R and Baquero, F. (2000). Streptococcus pyrogenes resistance to erythromycin in relation to macrolide consumption in Spain (1986-1997). J. Antimicrob. Chemother., 46: 959 - 964.
[45] Chandran, A. (2008). India Prevalence of Multiple Drug Resistant Escherichia coli Serotypes in a Tropical Estuary, India., Microb. Environ., 23( 2):153-158.
[46] Zinnha, M. A. (2008). Bangladesh, Drug sensitivity pattern of Escherichia coli isolated from samples of different Biological and environmental sources, Bangl. J. Vet. Med., 6(1): 13-18
[47] Chattopadhyay, U. K., Gupta, S. and Dutta, S. (2010). Search for shiga toxin producing Escherichia coli (STEC) including 0157: H7 strains in and around Kolkata., Ind. J. Med. Microbiol, 21(1), 17-20.
[48] Tesfaye, G., Astrat, D., Woldeamanuel, Y. and Gizaw, M. (2009). Microbiology of discharging ears in Ethiopia. Asian Pacific Journal of Tropical Medicine, 2(91): 60-67
[49] Abebe, M., Hailelule, A., Abrha, B., Nigus, A., Birhanu, M., Adane, H., Genene, T., Daniel, H., Getachew, G., Merga, G. and Haftay, A. (2014). Antibiogram of Escherichia coli strains from food of bovine origin in selected Woredas of Tigray, Ethiopia. Journal of Bacteriology Research, 6(3): 17-22.
[50] Wariso, B. A. and Ibe, S. N. (2006). Bacteriology of chronic discharging ears in Port Harcourt, Nigeria. West African Journal of Medicine, 25: 219-222.
Cite This Article
  • APA Style

    Nfongeh Joseph Fuh, Owoseni Mojisola Christiana, Obande Godwin Attah, Upla Peter Uteh, Odonye Dauda Dantani, et al. (2018). Risk Analysis and Antibiogram Spectrum of Escherichia coli O157: H7 Serotype from Children Stool and Raw Bovine Meat in Households Across Cross River State, Nigeria. European Journal of Clinical and Biomedical Sciences, 4(3), 39-45. https://doi.org/10.11648/j.ejcbs.20180403.11

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    ACS Style

    Nfongeh Joseph Fuh; Owoseni Mojisola Christiana; Obande Godwin Attah; Upla Peter Uteh; Odonye Dauda Dantani, et al. Risk Analysis and Antibiogram Spectrum of Escherichia coli O157: H7 Serotype from Children Stool and Raw Bovine Meat in Households Across Cross River State, Nigeria. Eur. J. Clin. Biomed. Sci. 2018, 4(3), 39-45. doi: 10.11648/j.ejcbs.20180403.11

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    AMA Style

    Nfongeh Joseph Fuh, Owoseni Mojisola Christiana, Obande Godwin Attah, Upla Peter Uteh, Odonye Dauda Dantani, et al. Risk Analysis and Antibiogram Spectrum of Escherichia coli O157: H7 Serotype from Children Stool and Raw Bovine Meat in Households Across Cross River State, Nigeria. Eur J Clin Biomed Sci. 2018;4(3):39-45. doi: 10.11648/j.ejcbs.20180403.11

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  • @article{10.11648/j.ejcbs.20180403.11,
      author = {Nfongeh Joseph Fuh and Owoseni Mojisola Christiana and Obande Godwin Attah and Upla Peter Uteh and Odonye Dauda Dantani and Fadayomi Victor Kolawole and Uchenwa Mercy Ogechi},
      title = {Risk Analysis and Antibiogram Spectrum of Escherichia coli O157: H7 Serotype from Children Stool and Raw Bovine Meat in Households Across Cross River State, Nigeria},
      journal = {European Journal of Clinical and Biomedical Sciences},
      volume = {4},
      number = {3},
      pages = {39-45},
      doi = {10.11648/j.ejcbs.20180403.11},
      url = {https://doi.org/10.11648/j.ejcbs.20180403.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejcbs.20180403.11},
      abstract = {This cross sectional study is aimed at evaluating the risk factors and antibiogram profiles of Escherichia coli O157:H7 in children stool and bovine meat obtained from selected households across Cross River State, Southern Nigeria. A total of 360 samples of fresh household bovine meat and 366 children diarrheal and nondiarrheal stool samples each were collected and examined for E. coli O157: H7 using standard culture and serological methods. Confirmed E. coli O157: H7 isolates were evaluated for antimicrobial susceptibility using the Agar disc diffusion method. The total positive samples for E. coli O157: H7 in household meat was 76/360 (21.11%,) while the diarrheaic and nondiarrheaic stool samples had 70/366 (19.13%) and 5/366 (1.37%) positive samples respectively. A significant difference was observed in the prevalence values among the bovine meat samples from various households and between the diarrheaic and nondiarrheaic samples at pAge range with highest prevalence value at 1-2yrs (26.83%); Occupation of parent/guardian with highest value from farming (25.67%) and Main domestic water source with highest value from surface water (28.21%) were observed to significantly affect the prevalence of the pathogen in children diarrheaic stool (pE. coli O157:H7 infection in children in the study communities with diarrheal stool being the main vehicle for secondary infections in humans. Cattle therefore serve as a major source of transmission of multi drug resistant E. coli O157:H7 to humans hence the need for continuous surveillance of this pathogen and implementation of legislation against indiscriminate use of antibiotics in diary farms.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Risk Analysis and Antibiogram Spectrum of Escherichia coli O157: H7 Serotype from Children Stool and Raw Bovine Meat in Households Across Cross River State, Nigeria
    AU  - Nfongeh Joseph Fuh
    AU  - Owoseni Mojisola Christiana
    AU  - Obande Godwin Attah
    AU  - Upla Peter Uteh
    AU  - Odonye Dauda Dantani
    AU  - Fadayomi Victor Kolawole
    AU  - Uchenwa Mercy Ogechi
    Y1  - 2018/08/24
    PY  - 2018
    N1  - https://doi.org/10.11648/j.ejcbs.20180403.11
    DO  - 10.11648/j.ejcbs.20180403.11
    T2  - European Journal of Clinical and Biomedical Sciences
    JF  - European Journal of Clinical and Biomedical Sciences
    JO  - European Journal of Clinical and Biomedical Sciences
    SP  - 39
    EP  - 45
    PB  - Science Publishing Group
    SN  - 2575-5005
    UR  - https://doi.org/10.11648/j.ejcbs.20180403.11
    AB  - This cross sectional study is aimed at evaluating the risk factors and antibiogram profiles of Escherichia coli O157:H7 in children stool and bovine meat obtained from selected households across Cross River State, Southern Nigeria. A total of 360 samples of fresh household bovine meat and 366 children diarrheal and nondiarrheal stool samples each were collected and examined for E. coli O157: H7 using standard culture and serological methods. Confirmed E. coli O157: H7 isolates were evaluated for antimicrobial susceptibility using the Agar disc diffusion method. The total positive samples for E. coli O157: H7 in household meat was 76/360 (21.11%,) while the diarrheaic and nondiarrheaic stool samples had 70/366 (19.13%) and 5/366 (1.37%) positive samples respectively. A significant difference was observed in the prevalence values among the bovine meat samples from various households and between the diarrheaic and nondiarrheaic samples at pAge range with highest prevalence value at 1-2yrs (26.83%); Occupation of parent/guardian with highest value from farming (25.67%) and Main domestic water source with highest value from surface water (28.21%) were observed to significantly affect the prevalence of the pathogen in children diarrheaic stool (pE. coli O157:H7 infection in children in the study communities with diarrheal stool being the main vehicle for secondary infections in humans. Cattle therefore serve as a major source of transmission of multi drug resistant E. coli O157:H7 to humans hence the need for continuous surveillance of this pathogen and implementation of legislation against indiscriminate use of antibiotics in diary farms.
    VL  - 4
    IS  - 3
    ER  - 

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Author Information
  • Department of Microbiology, Faculty of Science, Federal University Lafia, Lafia, Nigeria

  • Department of Microbiology, Faculty of Science, Federal University Lafia, Lafia, Nigeria

  • Department of Microbiology, Faculty of Science, Federal University Lafia, Lafia, Nigeria

  • Department of Microbiology, Faculty of Science, Federal University Lafia, Lafia, Nigeria

  • Department of Microbiology, Faculty of Science, Federal University Lafia, Lafia, Nigeria

  • Department of Microbiology, Faculty of Science, Federal University Lafia, Lafia, Nigeria

  • Department of Microbiology, Faculty of Biological Sciences, University of Calabar, Calabar, Nigeria

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