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Activity of Lysosomal Enzymes During Triple Combination Antihypertensive Treatment of Arterial Hypertension

Summary: The aim of the study has been to study the biochemical parameters of blood, the activity of lysosomal enzymes in models of arterial hypertension (AH) and assess the effectiveness of three-component antihypertensive treatment. Research materials and methods: The experiment has included 23 rabbits of Chinchilla breed weighing 2.5-3.0kg, which the AH model has been created in. Of these, 8 rabbits which have not received antihypertensive therapy (AHT) have made up the control group. After 4 weeks of model creation, AHT has been started in 15 rabbits of the main group within 1 month. Over the course of 8 weeks, systolic blood pressure (SBP) and diastolic blood pressure (DBP) have been measured in rabbits using a veterinary electronic tonometer. Along with this, the level of creatinine, urea, residual nitrogen in the blood plasma, the activity of the enzymes lactate dehydrogenase (LDH) and the MB fraction of creatine phosphokinase (CPK-MB) have been determined, ECG studies have been performed. All rabbits have been prescribed valsartan/amlodipine/hydrochlorthiazide (80/12.5/5mg and 160/25/10mg), choosing an individual dose depending on blood pressure once a day per os. Results: During the study, along with an increase in blood pressure, an increase in the level of creatinine, residual nitrogen and urine in the blood plasma has been revealed when creating a model of hypertension, which has been accompanied by an increase during the month. A decrease in the activity of the enzyme LDH and CPK-MB has been also noted. After the therapy hold for 1 month, positive dynamics have been found both in the level of blood pressure and indicators of biochemical analysis. Since creatinine concentration has decreased by 28.5%, residual nitrogen – by 36%, urea – by 40%, LDH activity has increased by 30%, CPK-MB by 40%. Conclusion: Thus, along with an increase in blood pressure and indicators of biochemical analysis, a decrease in the activity of lysosomal enzymes is noted in AP models. An increase in the activity of the enzymes LDH and CPK-MB during the three-component AHT shows that the components of this combination affect various parts of the cellular metabolism.

Experimental Models, Arterial Hypertension, Lysosomal Enzymes, Fixed Drug Combination

Azayeva Nurana Rafail Gizi. (2023). Activity of Lysosomal Enzymes During Triple Combination Antihypertensive Treatment of Arterial Hypertension. European Journal of Clinical and Biomedical Sciences, 9(4), 47-50.

Copyright © 2023 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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